A team of University scientists at has developed a microscope capable of live imaging at double the resolution of fluorescence microscopy using structured illumination.

The fluorescence microscope has allowed a generation of scientists to study the properties of proteins inside cells.

Yet, as human capacity for discovery has zoomed to the nanoscale, fluorescence microscopy has struggled to keep up.

The laws of physics have limited the resolution of fluorescence microscopy, whereby a fluorescent marker is used to distinguish specific proteins, to about 200 nanometers.

At this resolution significant detail is lost about the activity within a cell.

Now, the increased resolution by structured illumination is an engineering feat that will help scientists learn more about cell behavior and study mechanisms important for human disease.

“Our understanding of what is going on inside cells and our ability to manipulate them has advanced so much that it has become more and more important to see them at a better resolution,” said UGA engineer Peter Kner.

The importance of fluorescence microscopy was recently recognized with the 2008 Nobel Prize for Chemistry, which was awarded for the development of the green fluorescent protein, which has played a crucial role in our identification and understanding of proteins.

“What we’ve done is develop a much faster system that allows you to look at live cells expressing GFP, which is a very powerful tool for labeling inside the cell,” Kner explained.

- UGA News Service